Phospho flow antibodies
WebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: Akt1, Akt2, Akt3, PKBα, PKBβ, PKBγ, RAC-PKα, RAC-PKβ, RAC-PKγ, STK-2 Reactivity: Human (QC Testing), Mouse (Tested in Development) Isotype: Mouse BALB/c IgG1, κ Immunogen: Phosphorylated Human Akt1 (pS473) Peptide WebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: SMAD2, SMAD3; MADH2, MADH3; MAD homolog 2, MAD homolog 3 Reactivity: Human (QC Testing), Mouse (Tested in Development), Rat (Predicted) Isotype: Mouse IgG1, κ Immunogen: Phosphorylated Human Smad2 Peptide
Phospho flow antibodies
Did you know?
WebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques.26 Several phospho-specific antibodies have been developed for Raf-1, including antibodies that recognize phospho-Ser-338 and phospho-Tyr-340/341. WebTermed "phospho-flow," these methods employ fluorescence-conjugated monoclonal antibodies that recognize phosphorylated epitopes of intracellular kinases, and may be combined with surface phenotypic markers to observe changes in …
WebPhosphoserine Antibodies Antibodies that detect Phosphoserine can be used in several scientific applications, including Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation and Dot blot. Our Phosphoserine monoclonal and polyclonal antibodies are developed in Mouse and Rabbit. Find the Phosphoserine antibody that fits … WebFlow Cytometry - APC Anti-p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] (ab278728) Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278728.
WebFlow cytometry offers a quick and effective way to measure signaling at a specific time point in individual cells. The staining methods for detecting signaling molecules and … WebJan 1, 2012 · Antibodies: 5. Anti-human and mouse phospho-STAT3 (pY705) mouse antibody conjugated to phycoerythrin (PE) (BD/Phosflow, catalogue number: 612569). 6. Anti-human and mouse phospho-STAT5a,b (pY694) mouse antibody conjugated to Alexa Fluor ® 488 (BD/Phosflow, catalogue number: 612598). 7.
WebJun 5, 2015 · The eight phospho-specific antibodies were chosen to represent major signaling nodes responsive to cytokine, TLR, and antigen receptor signaling. This antibody …
WebPhosphoflow is a specialized application of flow cytometry that enables researchers to examine the phosphorylation status of specific proteins that govern cellular responses … bjt ppt downloadWebFor example, staining intracellular targets for phospho-flow cytometry experiments requires that you fix and permeabilize your cells before staining. However, fixation (fix) and permeabilization (perm) protocols can alter the interaction between epitope and antibody. bjt proportional base drive self-startingWebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. dating in seattle as a manWebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies … dating in san francisco as a mandating in scotland for over 50WebAnti-RhoA (phospho S188) antibody See all RhoA primary antibodies Description Rabbit polyclonal to RhoA (phospho S188) Host species Rabbit Specificity Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins. Tested applications Suitable for: WB more details Species reactivity Reacts with: Human dating in seattle is hardWebPrimary Antibodies (9010) Secondary Antibodies (76) Antibody Conjugates (1994) Activators & Inhibitors (224) Buffers & Dyes (108) Cellular Assay Kits (118) ChIP Kits & Reagents (156) CUT&RUN Kits & Reagents (15) Cytokines & Growth Factors (215) ELISA Kits (513) Experimental Controls (248) Flow Cytometry Kits & Reagents (9) Proteomic Analysis … bjt properties inc