Splet06. jun. 2024 · The purified extracted RNA was quantitatively measured by NanoDrop, at wavelengths of 260 and 280 nm. cDNA was constructed following the procedures: 2 μg of RNA utilizing of MMLV reverse transcriptase with oligo dt primers and random hexamer based on the manufacture protocol. Splet14. okt. 2024 · The aim of the present experiments was to optimise the process of DNA …
Establishment of a PCR Assay for the Detection and
Splet22. apr. 2024 · RNA Extraction and Semiquantitative RT-PCR. ... Lyophilized herbal extracts of different amounts were resuspended in saline buffer and orally administered to mice (200 μL) twice daily for 4 days. On day 4, 3 h after the administration of the herbal extracts, the mice were intraperitoneally injected with 10 mg/kg lipopolysaccharide ... SpletNational Center for Biotechnology Information boohoo office leicester
Development of real-time PCR based molecular markers for two
SpletEthanol extracts of Sanguisorba officinalis L. suppress TNF-α/IFN-γ-induced pro-inflammatory chemokine production in HaCaT cells [2015] ... â at the mRNA level was determined by real-time reverse transcription-polymerase chain reaction (RT-PCR). The ability of ESOL to reduce the expression of pro-inflammatory marker proteins was ... Splet25. avg. 2014 · Salmonella sp. could not be detected in DNA samples extracted from herbal medicines.Conclusion: We conclude that PCR and similar new molecular techniques such as Real Time PCR have the potential as rapid microbiological analytical techniques especially in busy clinical settings and for quality control of herbal medicines.Key words: … Splet25. avg. 2014 · In this study we investigated the ability to use Polymerase Chain Reaction … godinger modern vintage collection