Ntf2 mediates nuclear import of ran

Author: lajz

August undefined, 2024

WebFIG. 6. Karyopherin a (Kap60) releases karyopherin b (Kap95) from RanGTP. A, Ran that was ;40% GTP and ;60% GDP bound was immobilized on a CM5 sensor chip as described under “Experimental Procedures.” Injection of 100 mg/ml Kap95 resulted in binding of 500 RU to the Ran surface. B, Ran was immobilized as in A, and 90 RU of Kap95 were … WebNTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct NTF2–RanGDP …

NTF2 mediates nuclear import of Ran. (Q24533395) - wikidata.org

WebThe small GTPase Ran plays a central role in nucleocytoplasmic transport. Nuclear transport of Ran itself depends on nuclear transport factor 2 (NTF2). Here, we report … WebSingle molecule microscopy techniques allow to visualize the translocation of single transport receptors and cargo molecules or particles through nuclear pore complexes.These data indicate that cargo molecule import into the nucleus takes less than 10 ms and nuclear export of messenger RNA (mRNA) particles takes 50–350 ms, up to several … st joseph\u0027s catholic church salmon arm

Nuclear import of Ran is mediated by the transport factor …

WebRibbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1998) NTF2 mediates nuclear import of Ran. EMBO J 17, 6587–6598. CrossRef PubMed CAS Google Scholar Ribbeck K, Kutay U, Paraskeva E, Görlich D (1999) The translocation of transportin-cargo complexes through nuclear pores is independent of both Ran and energy. Web16 apr. 1999 · NTF2 mediates nuclear import of Ran NTF2 mediates nuclear import of Ran Ribbeck, Katharina; Lipowsky, Gerd; Kent, Helen M.; Stewart, Murray; Görlich, Dirk … Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 st joseph\u0027s catholic church sheringham

RGD Reference Report - NTF2 mediates nuclear import of Ran.

Sumoylation of the GTPase Ran by the RanBP2 SUMO E3 Ligase …

Web1 nov. 1998 · Europe PMC is an archive of life sciences journal literature. Search life-sciences literature (Over 39 million articles, preprints and more) Web8 mrt. 2024 · For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. st joseph\u0027s catholic church thameWeb31 dec. 1998 · The nuclear import of Ran is mediated by NTF2. (a) Digitonin-permeabilized BHK21 cells were incubated for 30 min at 25°C with buffer containing an energy … st joseph\u0027s catholic church salmon arm bc

"Web8 dec. 2024 · Binding of Ran to NTF2 is required for NTF2 to inhibit nuclear expansion and import of large cargo molecules. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells. Data suggest actin accumulation in senescent cells is due to the failure of RanGTP restoration with ATP deficiency and NTF2 accumulation, resulting in decreased … " - Ntf2 mediates nuclear import of ran

Ntf2 mediates nuclear import of ran

Web16 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct … Web2 okt. 2024 · Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and …

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WebRan is a small G protein that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein has also been implicated in the control of DNA synthesis and cell cycle progression, as mutations in Ran have been found to disrupt DNA synthesis. [8] Function [ edit] Ran cycle [ edit] WebRan function is modulated in a noncatalytic fashion by NTF2, a protein that mediates nuclear import of Ran-GDP. Here we characterize a novel component of the Ran …

WebNuclear import in digitonin-permeabilized cells in the absence of added factors was minimal. The addition of energy and nuclear transport factor 2 (NTF2) [5] was sufficient for the accumulation of Ran in the nucleus. An NTF2 mutant that cannot bind Ran [6] was unable to facilitate Ran import. A GTP-bound form of a Ran mutant that cannot bind ... WebHere we show that NTF2 is a mediator of Ran import into the nucleus and that NTF2 is rate limiting for Ran import into nuclei of permeabilized cells. Moreover, a direct …

WebRibbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1998) NTF2 mediates nuclear import of Ran. EMBO J 17: 6587–6598. PubMed Google Scholar Rosenblum SR, Pemberton LF, Blobel G (1997) A nuclear import pathway for a protein involved in tRNA maturation. J Cell Biol 139: 1655–1661. PubMed Google Scholar Weband probably also mediates translocation of the NTF2– RanGDP complex to the nuclear side of the NPCs. A direct NTF2–RanGDP interaction is crucial for this process, since point mutations that disturb the RanGDP–NTF2 interaction also interfere with Ran import. The subsequent nuclear accumulation of Ran also requires GTP, but not GTP ...

WebFurthermore, it has been shown that nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP. This review largely focuses on the issue concerning the …

WebThe experiment shows that this mutant Ran failed to accumulate in the nuclei, confirming that the direct NTF2–RanGDP interaction is crucial for Ran import. In addition, we can conclude that... st joseph\u0027s catholic church somers nyWebis recognized in the cytoplasm by NTF2 (nuclear transport fac- tor 2), which mediates its rapid translocation into the nucleus where the Ran guanine nucleotide exchange factor RCC1 (regu- st joseph\u0027s catholic church sandpoint idWebtemperature-sensitive NTF2 mutants are defective in nuclear protein import. Moreover, temperature sensitive mutants of the yeast Ran homolog Gsplp can be suppressed by overexpression of NTF2, sug gesting that Ran-NTF2 interaction is required for effective nuclear protein import in vivo (Wong et al., 1997). Whether NTF2 is also required for … st joseph\u0027s catholic church somers point nj st joseph\u0027s catholic church tilehurst readingWebRan is a small G protein that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein has also been implicated in the control of … st joseph\u0027s catholic church stockportWebWe conclude that NTF2 functions as a transport receptor for Ran, permitting rapid entry into the nucleus where GTP-GDP exchange mediated by RCC1 [7] converts Ran into its GTP … st joseph\u0027s catholic church warragulWeb7 feb. 2008 · These observations may provide an explanation how CapG nuclear import can occur when only Ran or NTF2 is added. Discussion. Nuclear accumulation of CapG in permeabilized cells does not result from passive diffusion because CapG import is inhibited when Nups are obstructed by WGA, mAb414 or importin β (45–485). st joseph\u0027s catholic church st joseph mn